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Fig. 3
Gβ1 and Gβ1like depletion in zebrafish embryos. Zebrafish embryos were injected with MO-Ctrl (4 ng), MO-Gβ1 (3 ng) and MO-Gβ1like (3 ng) singly or in combination (3 + 3 ng). (A) Immunoblot analysis of lysates prepared 72 hpf using specific antibodies against NDPK B, Gβ1 and Gγ2. β-actin served as loading and specificity control. (B) Relative NDPK activity (normalized to MO-Ctrl) was quantified as formation of 3H-GTP from 3H-GDP and ATP in lysates of embryos injected as indicated. Data are means ± SEM, n = 3, *, P < 0.05 vs. MO-Ctrl.
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