ZFIN Image: Patten et al., 2009, Fig. 2

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Figure Caption

Fig. 2

PKCγ is expressed in the Mauthner cell, and its activation leads to an increase in mEPSC amplitude. (A) Anti-3A10, anti-PKCα, anti-βII, and anti-γ immunoreactivity in zebrafish (n = 5). Anti-3A10 is a neurofilament marker that identifies Mauthner cells and is used as a positive control. Only anti-PKCγ labels the Mauthner cell. (Scale bar, 50 μM.) (B) Ten-minutes application of the active form of PKCγ to the Mauthner cell cytosol caused an increase in mEPSC amplitude (n = 5, P < 0.001); 10-min application of heat inactivated PKCγ had no effect (n = 4). Controls represent a recording of AMPA mEPSCs at the 10-min time point in normal intra and extracellular solutions. (C) Intracellular application of γV5-3 (10 nM) blocked the PMA-induced increase in the amplitude (n = 6), whereas the control peptide (C1; 10 nM) had no effect (n = 4). (D) Immunoblot analysis of cytosolic and membrane fractions from zebrafish brain after incubation with or without γV5-3 and PMA (5 nM); γV5-3 inhibited the PMA-induced loss of PKCγ from the cytosolic fraction (n = 4, P < 0.001). ***, significantly different, P < 0.001.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA