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Fig. 2 Foxd3 is necessary for pnp4a expression. (A,B) Wild-type fish co-stained with pnp4a and Foxd3, 24 hpf, anterior trunk. Green: pnp4a mRNA, red: Foxd3 antibody (A) Punctate, cytoplasmic pnp4a mRNA signal surrounds Foxd3 positive nuclei, 63×. (B) Field reveals a pnp4a+/Foxd3+ cell (arrow) adjacent to three pnp4a-/Foxd3+ cells (*), 40x. (C,D) Flat mounted head and trunk stained with pnp4a riboprobe, 22 hpf, dorsal view, anterior left, 10x. (C) wild-type (D) foxd3-/- mutant (sym1). Scale bars: (A) 10 μm; (B) 20 μm; (C,D) 70 μm.
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Reprinted from Developmental Biology, 344(1), Curran, K., Lister, J.A., Kunkel, G.R., Prendergast, A., Parichy, D.M., and Raible, D.W., Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest, 107-118, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.