ZFIN Image: Atzei et al., 2010, Fig. 2

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Figure Caption

Fig. 2 Expression of zCactin during early embryonic developmental stages. (A) Total RNA (50 ng) was isolated from staged embryos and analyzed by RT-PCR using specific primers for zCactin. β-actin mRNA expression was determined as a control housekeeping gene. Sizes of zCactin and β-Actin fragments are 500 and 322 bp, respectively. Results are indicative of three independent experiments. (B) In situ hybridisation shows zCactin mRNA expression at a variety of embryonic developmental stages. zCactin is expressed from 8-cell stage to 48 hpf, where it strongly presents in the eyes. The sense probe was used as a negative control. Images were acquired using a Zeiss Axioplan II microscope and processed using Adobe Photoshop.

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Expression / Labeling details

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 10(4-5), Atzei, P., Yang, F., Collery, R., Kennedy, B.N., and Moynagh, P.N., Characterisation of expression patterns and functional role of Cactin in early zebrafish development, 199-206, Copyright (2010) with permission from Elsevier. Full text @ Gene Expr. Patterns