ZFIN Image: Brown et al., 2010, Fig. S5

Image

Figure Caption

Fig. S5 Quantification and controls for nlcam over-expression and morpholino
A: Quantification of the frequency of phenotypes observed upon injection of nlcam mRNA at 10ng/μl. Results show one representative experiment (ie embryos from a single batch injected at the same time). “Severe phenotype” denotes failed or disrupted gastrulation, or severe convergent-extension and/or neural tube defects.
B: Quantification of the frequency of phenotypes observed upon injection of nlcam ATG morpholino at either 0.2mM or 0.4mM (as indicated). As above, results show one representative experiment.
C, D: nlcam ATG morpholino represses GFP expression from a construct in which the target sequence is placed upstream of the GFP coding sequence. In embryos co-injected with this construct plus the morpholino, no GFP expression can be seen.
E, F: A second morpholino, targeted against the e3, i4 splice junction, phenocopes the ATG morpholino.

Acknowledgments

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Reprinted from Developmental Biology, 339(1), Brown, K.E., Keller, P.J., Ramialison, M., Rembold, M., Stelzer, E.H., Loosli, F., and Wittbrodt, J., Nlcam modulates midline convergence during anterior neural plate morphogenesis, 14-25, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.