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Fig. 4 Chemical and genetic knockdown of chondroitin sulfate prevents the proper formation of the atrioventricular (AV) canal in zebrafish. A: Wild-type Danio rerio heart at 55 hours postfertilization (hpf). B: The 55 hpf animals treated from 7-48 hpf with DX (cis/trans-decahydro-2-napthol-β-D-xyloside), an inhibitor of CS formation. C: 55 hpf animals injected with a morpholino to chondroitin synthase-1 (chys-1). at. atrium; v, ventricle; av, atrioventricular canal forming region.
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