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Fig. 2 gata5 and gata6 rescue and gain-of-function experiments confirm necessity and identify sufficiency for myelopoiesis. Injection of exogenous gata5 and gata6 mRNA (g5+6RNA) into wild-type embryos did not induce fli1 or etsrp, whereas scl and pu1 were ectopically expressed. Rescue of all four genes was seen in gata5 and gata6 morphants co-injected with gata5 and gata6 mRNA (g5+6mo + g5+6RNA) when compared with gata5 and gata6 morphants alone (g5+6mo). Views are anterior-dorsal.
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