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Fig. 2 Spatio-temporal expression patterns and knockdown analyses of foxi3a and foxi3b. All panels are lateral views, dorsal side up, anterior to the left. (A–F) Whole mount in situ hybridization with foxi3a probe (A–C) and foxi3b probe (D–F). Expression of foxi3a begins earlier than that of foxi3b (A, D). foxi3b-positive cells are distributed in the dorsal trunk region in addition to the ventral trunk region (F). (G, H) Expression of marker genes for vH-MRC (atp6v1a) and NaK-MRC (atp1a1a.2). (I–K) Double immunostaining with anti-vH-ATPase and anti-NaK-ATPase antibody at 48 hpf of uninjected wild-type control (I), foxi3a morphant (J), and foxi3b morphant (K). (L, M) Immunostaining with anti-Foxi3b antibody at 24 hpf of uninjected wild-type control (L) and foxi3b morphant (M). Knockdown of foxi3b with MO exerted no or little effects on the generation of vH-MRC and NaK-MRC (K) while knockdown of foxi3a resulted in complete loss of both vH-MRC and NaK-MRC (J).
Reprinted from Developmental Biology, 329(1), Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S., Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae, 116-129, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.