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Fig. 1 Distribution of two major types of MRC in the skin of zebrafish larvae and a mutant lacking one of them. (A) Double immunostaining of NaK-ATPase (magenta) and vH-ATPase (green) at 48 hpf, viewed from lateral side. (B–E) Phenotypes of quadro (quo) mutant. Defects are seen in otic and jaw development in quo mutant embryos (C, E). All panels show lateral views of live embryos with anterior to the left. j, jaw; ov, otic vesicle. (F, G) Double immunostaining with anti-vH-ATPase and anti-NaK-ATPase antibody at 48 hpf of wild-type control (F) and quo mutant (G).
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Reprinted from Developmental Biology, 329(1), Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S., Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae, 116-129, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.