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Fig. 8 Olig1 knockdown alone does not interfere with oligodendrocyte development unless combined with an Olig2 sensitized background. A: Injection of 750 μM olig1 morpholino (olig-1-MO) alone had no effect on mpb or plp expression at 96 hpf while 250 μM olig2-MO abolished mbp expression in the CNS. B: In contrast, co-injection of 750 μM olig1-MO with low concentrations of olig2-MO (75 μM), each morpholino individually having no phenotype, reduced mbp+ oligodendrocytes. Numbers in columns indicate numbers of observed embryos while n indicates total number of analyzed embryo per treatment/injection.
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