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Fig. 4 Slow-twitch muscle specification and subsequent migration is not required for localization of laminin, Fak and Fn at the MTJ during muscle morphogenesis, however expression is sometimes disrupted. All panels were obtained using a Zeiss ApoTome. Side views, anterior left, dorsal top of 24 hpf embryos. (A) Laminin (yellow) is highly concentrated at the MTJ of wild-type embryos (A, white arrow). In smu embryos, there are frequently gaps in the myotome boundary, and laminin often congregates at these gaps (A1, arrowheads) in addition to the MTJ (A1, arrow). (B) pY397 FAK (blue) is concentrated at the MTJ of wild-type embryos (B, white arrow). In smu embryos, pY397 FAK concentrates at the MTJ (B1, white arrow) but not at gaps (B1, yellow arrowheads). (C) Fn (green) is concentrated at the lateral MTJ of wild-type embryos (C, white arrow). In smu embryos, Fn concentrates at the MTJ (C1, white arrow) but is sometimes reduced/absent (C1, green arrowhead).
Reprinted from Gene expression patterns : GEP, 9(1), Snow, C.J., and Henry, C.A., Dynamic formation of microenvironments at the myotendinous junction correlates with muscle fiber morphogenesis in zebrafish, 37-42, Copyright (2009) with permission from Elsevier. Full text @ Gene Expr. Patterns