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Fig. S4 The reduction of FGF activity in Dlx3b/4b morphant embryos is transient, and begins to return to control levels by mid-somitogenesis. 12-somite embryos stained with riboprobes against erm, fgfr1, fgfr2, fgfr3, or fgfr4 show that expression is reduced in Dlx3b/4b morphant embryos (B,D,F,H,J) when compared to wild-type embryos (A,C,E,G,I). The asterisk marks the MHB. fb, forebrain; mb, midbrain; hb, hindbrain; r1, rhombomere 1; r2, rhombomere 2; r4, rhombomere 4; r6, rhombomere 6; som, somites; psm, presomitic mesoderm. (A–J) 12-somite embryos; lateral views with anterior to the left.
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Reprinted from Developmental Biology, 325(1), Esterberg, R., and Fritz, A., dlx3b/4b are required for the formation of the preplacodal region and otic placode through local modulation of BMP activity, 189-199, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.