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Fig. S5 Ca2+ green imaging of intracellular Ca2+ in zebrafish embryos. Pseudocolour of fluorescent images of Ca2+ signal in posterior mesoderm near the Kupffer's vesicle of control, gpr161 knockdown, ncx1-RNA rescue and pmca-RNA rescue of gpr161 knockdown embryos at 14 hpf. Each group contains 18 different embryos organized as decreasing order of Ca2+ green intensity. All scale bars were 100 μm.
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Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.