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Fig. S3 Early-born trigeminal sensory neurons contribute to various subpopulations independently of late-born neurons. Subpopulation markers were analyzed in embryos lacking late-born trigeminal sensory neurons. (A-D) Wild-type embryos were incubated from 24 hpf to 72 hpf with 2% DMSO alone (A,C) or 20 mM hydroxyurea and 150 μM aphidicolin (B,D). Treated embryos were fixed at 72 hpf and stained by in situ hybridization for p2x3a (A,B) and trpv1 (C,D). Side view, anterior towards the left. Scale bar: 10 μm.
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