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Fig. 8 shh over-expression leads to an increase in enteric neurons. Dorsal (A, B) and lateral (C, D) views of phox2b in enteric ganglia of 72 hpf control (A, C) and shh injected embryos (B, D). (E) Bar graph summarizes the results from one of three independent shh injection experiments. Enteric ganglia from shh injected embryos contained more phox2b expressing cells around the gut (P = 2.7e - 05; Student's t-test). The error bars represent the standard deviation. Arrows and arrowheads (A–D) indicate phox2b expressing ENS precursors.
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Reprinted from Developmental Biology, 318(1), Reichenbach, B., Delalande, J.M., Kolmogorova, E., Prier, A., Nguyen, T., Smith, C.M., Holzschuh, J., and Shepherd, I.T., Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish, 52-64, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.