Fig. S1

Fig. S1 The asymmetric distribution of plasma membrane proteins is maintained in tjp3/zo-3 morphants. Epithelial cell polarization in the pronephric ducts. Immunofluorescence on cryosections: anti-Tjp3/Zo-3 (green) and anti-E-cadherin (red) (a-f); anti- PKCζ (green) and anti-Na+/K+-ATPase (red) (g-l). Control morpholino at 1 dpf (a, g) and 2 dpf (d, j); tjp3/zo-3 splice morpholino at 1 dpf (b, h) and 2 dpf (e, k) and tjp3/zo-3 translation blocking morpholino at 1 dpf (c, I) and 2 dpf (f, l). Note the strong reduction of Tjp3/Zo-3 protein from the apical side of the pronephric ducts in morphants (arrows), whereas E-cadherin or Na+/K+- ATPase staining on the basolateral membrane or PKCζ labeling on the apical membrane is not affected.