Fig. 6

Fig. 6 Ripply genes modulate the RA response of Mesp genes. (A) Zebrafish embryos treated for 2 h with RA and/or CHX as indicated and stained for the expression Ripply1 (top row) or Ripply2 (bottom row). Anterior is oriented up for zebrafish embryos. (B) Xenopus embryos treated for 1.5 h with RA and/or CHX as indicated and stained for the expression of bowline (top row) or ledgerline (bottom row). Anterior is oriented to the left for frog embryos. (C–E) Zebrafish transgenic for Xl-mespb-GFP embryos were injected with the indicated morpholino: R1 = anti-Ripply1; R2 = anti-Ripply2; R1 + 2 = both morpholinos. Injected embryos were treated for 4 h with DMSO (carrier control) or with RA and then stained for expression of C: mespb; D, mespa; or E, GFP. Anterior is up in all zebrafish panels. (F–I) Xenopus embryos injected with bowlineth RA and then stained for expression of C: mespb; D, mespa; or E, GFP. Anterior is up i (bln, F), ledgerline (ldg, G), both bln and ldg (H), or lacZ RNAs (I). Embryos were treated with DMSO or RA (top and bottom, respectively) and stained for localization of Xl-mespb transcripts. Within each boxed area, the two sides of a single embryo are shown. The uninjected side serves as an internal control and is always shown on the left side of the box.