Fig. 4

Fig. 4 plcβ3 is expressed in the pharyngeal arches and in neuronal cells. Lateral views of in situ hybridizations in wild-type embryos at 24 (A, C, E, F) and 28 hpf (B, D). Anterior is to the left. At 24 and 28 hpf, plcβ3 is expressed in a first arch domain adjacent to the first pouch (A, B), similar to a subset of the islet1 trigeminal ganglion domain (D). At 24 hpf, plcβ3 is also expressed in the second arch (A), similar to the dlx2a neural crest domain (C). At 28 hpf, plcβ3 is only weakly expressed in the second arch neural crest domain (B). Lateral trunk views at the same axial level of plcβ3 (E) and islet1 (F) RNA in situ hybridizations with wild-type embryos at 24 hpf. plcβ3 is expressed in a subset of cells at appropriate positions to be Rohon–Beard spinal sensory neurons. a1 and a2 label pharyngeal arch 1 and 2. Dotted lines label pharyngeal pouch 1. Arrowheads label cells at position of trigeminal ganglion domain. Scale bar: 50 μm.