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Fig. 1 Gene Expression Patterns Divide the ALPM into Three Territories (A–K) Two-color fluorescent in situ hybridization of wild-type embryos at the 7 somite stage, dorsal views, anterior to the top. All images are shown at the same magnification; the scale bar represents 100 μm. (A) Overlay of gata4 (B) and hand2 (C) expression. Expression of hand2 is observed throughout the caudal portion of gata4-expressing ALPM, but is excluded from the rostral ALPM. (D) Overlay of gata4 (E) and nkx2.5 (F) expression. Expression of nkx2.5 is observed within a medial portion of the caudal ALPM, but is excluded from the lateral and rostral ALPM. (G) Overlay of gata4 (H) and scl (I) expression. Expression of scl is observed in the rostral ALPM. (J) Overlay of hand2 (green) and scl (red) expression. Expression patterns of scl and hand2 do not overlap. (K) Overlay of hand2 (green) and nkx2.5 (red) expression. Expression of hand2 extends beyond the lateral limit of nkx2.5 expression. (L) Schematic of 7 somite stage ALPM, superimposed onto a dorsal view of a live embryo; dashes outline the tip of the notochord. Comparison of gene expression patterns defines rostral (white), medial (black), and lateral (gray) ALPM territories. Although ALPM morphology varies slightly during the 6–9 somite stage and between individual embryos, relative patterns of gene expression consistently demarcate these territories.
Reprinted from Developmental Cell, 13(2), Schoenebeck, J.J., Keegan, B.R., and Yelon, D., Vessel and blood specification override cardiac potential in anterior mesoderm, 254-267, Copyright (2007) with permission from Elsevier. Full text @ Dev. Cell