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Fig. S2 Retinoic acid pathway is not affected in cdx1a/cdx4-deficient embryos at the end of gastrulation. Expression of genes encoding the retinoic acid (RA) -synthesizing enzyme Raldh2 (also known as Aldh1a2 − ZFIN) (A,D), Retinoic acid receptor α (RARα; also known as Raraa − ZFIN) (B,E) and the RA-degrading enzyme Cyp26a1 (C,F) in wild-type (A-C) and cdx1a/cdx4-deficient embryos (D-F) at the end of gastrulation (tailbud stage, 10 hpf). Expression of these genes in the paraxial mesoderm, head and tail regions of cdx1a/cdx4-deficient embryos is similar to that seen in wild-type siblings. More than 40 embryos from two independent experiments were analyzed, all displaying the expression patterns shown. Embryos are shown in dorsal (A,D) or lateral (B,C,E,F) views, anterior to the top. Scale bar: 100 μm.