Fig. 3
Fig. 3 Reduction in Brg1 expression leads to aberrant expression of genes involved in neurodevelopment. In situ hybridization analyses showing the expression of various markers in control-MO-injected (10 ng/embryo) or Brg1-MO-injected (10 ng/embryo) embryos at indicated time points. The markers were as follows: six3 (A,B), eng2 (C-F), krox-20 (G-J), myoD (K,L), gsc (M,N), ntl (O-R), and hlx1 (S,T). Morphant phenotypes were more than 90%, n = 40. Embryos are dorsal view (A-P) or lateral view (Q-T). For in situ hybridization analyses, a total of 3,000 embryos was injected and 200 embryos were used per probe. No nonspecific hybridization was observed with sense probes (data not shown). Embryos were photographed using a SPOT camera mounted on a dissecting microscope. Morphant phenotypes were more than 90%, n = 30. Original magnification 40×.