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Fig. 4 Regulation of AV endocardial development. Confocal images of the AV canal of wild-type (A) and mutant (B) hearts at 55 hpf. (A,B) Tg(flk1:EGFP)s843 (pseudo-colored blue) embryos immunostained for Dm-grasp (pseudo-colored green) and stained with rhodamine phalloidin (red). In contrast to wild type (A), in sih mutant embryos (B), AV canal endocardial cells (arrowhead) fail to express Dm-grasp and to adopt a cuboidal shape. Interestingly, the sih mutant myocardium is devoid of filamentous actin staining. A, atrium; V, ventricle.
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