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Fig. S3 Rescue of IFT morphant phenotype by hippi mRNA injection. Overexpression of zebrafish hippi by injection of capped RNA (2.3 ng) does not cause a visible phenotype, but is capable of rescuing the phenotype caused by injection of 4.6 nl of 0.1 mmol/l hippi SP morpholino. All 55 morpholino-injected embryos showed pronephric cyst formation (arrow and * in histological cross section) and some degree of hydrocephalus (arrowhead and ** in histological cross section), with 48 of 55 also having a ventrally bent body axis, whereas 6 appeared normal. Of the 44 co-injected embryos, 4 had a bent body axis, and hydrocephalus, but no renal cyst formation. Four had a straight body axis and no hydrocephalus, but kidney cysts, and 36 appeared wild type, which was confirmed by histology.