Amiodarone restricts Mmar infection in a host-directed manner. (A) Bacterial burden assay of mWasabi-expressing Mmar-infected zebrafish larvae treated with increasing doses of amiodarone (5, 10, and 20 µM) or vehicle control dimethyl sulfoxide (DMSO). Treatment was started at 1 hpi. and larvae were anesthetized at 4 dpi for imaging. Representative stereo fluorescent images of whole larvae infected with mWasabi-expressing Mmar. Magenta shows Mmar. Scale bar annotates 1 mm. (B) Quantification of bacterial burden shown in panel A. Bacterial burden was normalized to the mean of the control. Data from two independent experiments were combined (n = 39–42 per group). Boxplots with 95% confidence intervals are shown, and the black line in the boxplots indicates the group median. Statistical significance was tested using a Kruskal-Wallis with Dunn’s multiple comparisons test. (C) Bacterial burden assay of mWasabi-expressing Mmar-infected zebrafish larvae treated with 5 µM of amiodarone or vehicle control DMSO. Treatment was started at 1 hpi, and larvae were anesthetized at 1, 2, 3, and 4 dpi for imaging. Bacterial burden was normalized to the control (DMSO at 1 dpi), and data from two experimental repeats were combined (n = 65–70 per group). Boxplots with 95% confidence intervals are shown, and the black line in the boxplots indicates the group median. Statistical significance was tested using a Kruskal-Wallis with Dunn’s multiple comparisons test. ns, non-significant; *P < 0.05; **P < 0.01; and ****P < 0.0001.
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