Functional analysis reveals late onset muscle weakness in double dnajb6 mutants. (A) The distance travelled was not significantly different between genotypes at 6 dpf. The swimming of dnajb6 fish was recorded for a period of 10 min and each dot represents an individual fish. Analysis was completed in triplicate with n ≥ 38 per genotype. Error bars show standard error of the mean (SEM). Genotypes were compared using a linear model. (B) Double mutant fish show a significant decline in motor function over time (P = 0.007). Fish were genotyped prior to the start of the experiment and their movement was recorded weekly from 2 weeks post fertilisation (wpf) to 12 wpf. Each dot represents the average swimming distance of one group, with six groups per genotype. Analysis was completed with n ≥ 34 per genotype from week 2 onwards and a mixed linear model. (C) Survival of double mutant fish was significantly less than wildtype fish (P = 0.0005). Analysis was completed with n ≥ 29 per genotype from week 1 onwards. Kaplan–Meier survival curves were compared using the mantel-cox log rank test.
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