Assessment of color-associated learning and memory in the plus-maze test. A: Schematic diagram of plus-maze and representative swimming trajectories in the plus-maze test. B: Quantitative analysis of the ratio of distance swam in the blue area to the total distance swam in 2 dpf zebrafish after exposure to four different combinations of AlCl3 and D-gal for 72 h. Data are presented as the mean ± SEM (n = 30 zebrafish larvae per group), and the distance of five zebrafish represents one data point. Statistical significance of treatment effects was assessed using one-way ANOVA with Dunnett’s multiple comparison test and illustrated as follows: * * p < 0.01 (versus the control group).
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