Fig. 2
- ID
- ZDB-FIG-240528-89
- Publication
- Mandal et al., 2024 - Caged Dexamethasone to Photo-control the Development of Embryos through Activation of the Glucocorticoid Receptor
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cDEX-A photoactivation in Ventx-GR-injected zebrafish and Xenopus embryos. Whereas the zebrafish (a) and Xenopus (d) embryos injected with Ventx-GR mRNA and incubated in the dark with 15 μM cDEX-A at 4 hpf (a,b; inset; scale bar: 100 μm) and blastula stage (d,e; inset; scale bar: 500 μm) exhibit normal phenotypes at 24 hpf and tailbud stage respectively, upon illumination at 365 nm for 600 s these zebrafish (b) and Xenopus (e) embryos reveal strong phenotypes at 24 hpf (b) and tailbud stage (e); c,f: Extent of Ventx-GR-induced phenotype determined at 24 hpf (c) and tailbud stage (f) in zebrafish (c) and Xenopus (f) embryos incubated (+) or not (−) at 4 hpf (c) and blastula stage (f) with 7 or 15 μM DEX or cDEX-A and illuminated (+) or not (−) at 4 hpf (c) and blastula stage (f) at 365 nm with 5.7×10−4 E.m−2.s−1 for 600 s (see Tables S3 and S4 for zebrafish and Xenopus embryos respectively). Bars: Extent of phenotypes C–I (dark cyan), C–II (orange), and C–III (dark red) (see Figures S3 and S4 and Tables S3 and S4). T=293 K. |