FIGURE

Fig. 3

ID

ZDB-FIG-240402-8

Publication

Liang et al., 2024 - A novel gene-trap line reveals the dynamic patterns and essential roles of cysteine and glycine-rich protein 3 in zebrafish heart development and regeneration

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Fig. 3

csrp3 expression increases in response to zebrafish heart injury. A The temporal expression profiles of GFP in + /218A hearts (upper) and endogenous csrp3 in wild-type hearts (bottom) after ventricle ablation. Scale bars, 20 μm. B Immunostaining showed the colocalization of GFP fluorescence with MF20-marked myocardium in + /218A larval hearts after ventricle ablation. Scale bar, 20 μm. C Confocal images illuminated a significant increase of GFP fluorescence in TnnT2(CT3)-marked myocardial cells at the border zone of the cryoinjured + /218A adult ventricle. Areas of dashed boxes are magnified. Scale bars, 100 μm. D Immunostaining revealed that GFP fluorescence of + /218A was specifically expressed in myocardial (Actn1⁺) cells, while absent in the endocardial (Flk⁺) and epicardial (Pck⁺) cells. Areas of dashed boxes are magnified. Scale bars, 100 μm. E Fluorescence in situ hybridization (FISH) analysis corroborated a robust upregulation of csrp3 expression at the border zone of cryoinjured ventricle, compared to the faint and dispersive signal in the uninjured adult ventricle. Scale bars, 100 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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