Fig. 1

Sorbs1 expression is enriched in the endothelium and its deletion in zebrafish results in cardiac edemas. A Transmitted light images of live wild-type (WT) and sorbs1 mutant (sorbs1^−/−) zebrafish embryos at 5 days post-fertilization (dpf). The white arrow indicates an example of edema observed in sorbs1^−/− embryo. Proportions of embryos with edemas in each genotype are indicated. Scale bar represents 250 μm. B Quantification of the percentage of survival for sorbs1^−/− embryos presenting or not edemas from 4 to 10 dpf (n = 23 and n = 47, respectively). C Quantification of the heart rate in WT and sorbs1^−/− embryos at 48 hpf (n = 45 and n = 49, respectively). D Illustration of the procedure used to sort endothelial cells by FACS (fluorescence-activated cell sorting) from Tg(fli1a:eGFP)y1 zebrafish embryos, at different stages of development, followed by RNA extraction and reverse transcription-qPCR (RT-qPCR). E, F RT-qPCR analysis of sorbs1 expression relative to Elfa after FACS sorting as described in D, in endothelial cells (ECs, GFP + , green bars) vs non-endothelial cells (non-ECs, GFP-, purple bar) (E) or in endothelial cells at different time points of embryonic development (F) (*P < 0.05, unpaired t-test). G UMAP of sorbs1 expression at single-cell level obtained from the Daniocell database consisting of whole-animal wild-type zebrafish across multiple stages of development (14–120 hpf). Cluster annotation was taken from the Daniocell resource