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The locomotion and thigmotaxis behavior of 7-dpf WT and mutant zebrafish. (A) Process of light/dark test of larval nomo1+/+ and nomo1−/− zebrafish at 7 dpf. The whole experiment lasted for 75 min and consisted of 40 min of light (L0) and two 5-min light/dark cycles (L1-D1 and L2-D2). (B) Trend of total distance swum by larval zebrafish during the experiment. The horizontal axis is the swimming time, and the vertical axis is the distance moved to visualize the locomotion of zebrafish during the experiment (biological replicates, N = 48). (C) The average distance moved per minute under continuous illumination is shown (biological replicates, N = 48 per genotype). (D, E) The average distance moved per minute under two light/dark cycles (biological replicates, N = 48 per genotype). (F) Ratio of the distance moved by larval zebrafish during each light/dark cycle (biological replicates, N = 48 per genotype). (G) The inner and outer zones were delineated as shown in 24-well plate. The ratio of distance moved/time spent in the inner zone were to measure the frequency of thigmotaxis behavior. (H) The ratio of distance moved/time spent in the inner zone under light conditions (L0 period) in 7-dpf zebrafish larvae (N = 48). Data information: Data are analyzed using unpaired t test and presented as the means ± SEM, *P < 0.05, **P < 0.01, ****P < 0.0001. Source data are available online for this figure.
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