FIGURE

Fig. 2

ID

ZDB-FIG-240201-57

Publication

Ugajin et al., 2023 - Znf598-mediated Rps10/eS10 ubiquitination contributes to the ribosome ubiquitination dynamics during zebrafish development

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Fig. 2

Detection of ribosome ubiquitination under normal and stress conditions. (A) Detection of ribosome ubiquitination. Total lysates (input) and FLAG-IPs obtained from WT and rpl36-FLAG embryos at 24 hpf were subjected to immunoblotting analysis with an anti-Ubiquitin antibody (left) and protein staining (right). (B) Analysis of ribosome ubiquitination under harringtonine (HTN) treatment. FLAG-IPs were subjected to immunoblotting analysis with an anti-Ubiquitin antibody (left) and protein staining (right). HTN concentrations are indicated above. (C) Analysis of ribosome ubiquitination under cycloheximide (CHX) treatment. FLAG-IPs were subjected to immunoblotting analysis with an anti-Ubiquitin antibody (left) and protein staining (right). CHX concentrations are indicated above. (D,E) Analysis of ribosome ubiquitination in the presence of mutant release factor eRF1-AAQ. Total lysates were subjected to immunoblotting analysis of eRF1-HA and Tubulin (D). FLAG-IPs were subjected to immunoblotting analysis with an anti-Ubiquitin antibody (E, left) and protein staining (E, right).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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