Effect of the −2055~+77 DNA fragment of human ENDOU-1 gene on reporter gene expression in stress conditions. (A). Histograms presenting the relative luc activity obtained from HEK-293T cells co-transfected with pE2.1p (100 ng/well) and phRG-TK (20 ng/well) and then treated with either DMSO (control) or Thapsigargin (TH; stress inducer) for 2 or 6 h. HEK-293T cells treated with DMSO served as the control group. The relative luc activity was represented by the fold increase of Flu/Rlu ratio over that obtained from the control group normalized as 1. Values representing DMSO- and TH-treated cells were calculated from four independent experiments and presented as mean ±SD (n = 4). Student’s t-test was used to determine significant differences between each group (**, p < 0.05; ***, p < 0.005). (B). Histograms presenting the relative luc activity obtained from zebrafish embryonic cells microinjected at the one-cell stage with pE2.1p (6.9 pg/embryo) combined with phRG-TK (2.3 pg/embryo), followed by heat shock at 72 hpf and luc quantification at 96 hpf. Zebrafish embryos treated with microinjection, but not treated with heat shock, served as the control group. The relative luc activity was represented by the fold increase in the Flu/Rlu ratio over that obtained from the control group normalized as 1. Each value was averaged from three independent experiments and presented as mean ± SD (n = 3). Student’s t-test was used to determine significant differences between each group (***, p < 0.005).
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