FIGURE

Fig. 8

ID

ZDB-FIG-231215-199

Publication

Kim et al., 2023 - Nutlin-3a induces KRAS mutant/p53 wild type lung cancer specific methuosis-like cell death that is dependent on GFPT2

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Fig. 8

Anticancer effects of nutlin-3a confirmed in vivo. A Representative confocal images of CM-Dil-labeled cancer cells (red) and vasculature (green) in zebrafish larvae (left). Quantified data show cancer cell volume after nutlin-3a treatment and GlcNAc co-treatment compared with that of the control group (right). Supplement of GlcNAc-rescued cancer cell volume reduces after nutlin-3a treatment. B, C Images (B) and comparison (C) of growth rates of subcutaneous tumors formed by A549 injection; tumor growth is observed in the presence or absence of nutlin-3a. D Weight of mice after treatment with either vehicle control or nutlin-3a on the day of euthanasia. E Immunohistochemical analysis of Ki67 expression in tumor tissues. F Vacuole examination in H&E stained tumor tissues. Red arrows indicate vacuoles. G Western blot analysis of GFPT2 and p53 expression in in vivo xenograft tumors. The densitometry quantification of the western blot was determined using Image J software (Ver. 1/52n, NIH). H Proposed mechanism of nutlin-3a-induced methuosis-like cell death. Scale bar: 20 μm for (A) and 50 μm for (E, F). Data are presented as mean ± standard deviation; n = 6. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Exp. Clin. Cancer Res.