FIGURE

Fig. 1

ID

ZDB-FIG-231215-188

Publication

Zhao et al., 2022 - The ETS transcription factor Spi2 regulates hematopoietic cell development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Phylogenic analysis and early expression pattern of spi2. (A) spi2 expression in the roof and floor of the dorsal aorta (DA) at 21 hpf and 28 hpf by scRNA-seq. ECs, endothelial cells. (B) Analysis of spi2 gene ortholog (1:1) during vertebrate evolution. Denominator: the number of analyzed species. Numerator: the number of species containing gene ortholog of spi2. (C) Phylogenic tree analysis of SPI-subfamily in zebrafish and mice. (D) Protein sequence alignment of ETS domain among spi2 paralogs in zebrafish. Asterisks indicate conserved amino acids among spi2 paralogs. (E) spi2 WISH at 24 hpf, 2 dpf and 3 dpf. Black arrowheads show spi2-expressing cells. (F) spi2 FISH and anti-GFP antibody staining in the aorta-gonad-mesonephros (AGM) of Tg(flk1:eGFP) zebrafish (white arrowheads: flk1+spi2+ cells) and in the caudal hematopoietic tissue (CHT) of Tg(cd41:eGFP) zebrafish (white arrowheads: cd41+spi2+ cells). (G) Pie chart shows the quantification percentage (±s.d.) of spi2+flk1+ cells in flk1+ cells in the floor of the DA at 2 dpf (fish n=5) or spi2+cd41+ cells in cd41+ cells in the CHT at 3 dpf (fish n=9) in F. Scale bars: 60 μm.

Expression Data

Gene:	spi2
Fish:	la2Tg/la2Tg s843Tg/s843Tg AB
Anatomical Terms:	caudal hematopoietic tissue dorsal aorta basal side endothelial cell ventral wall of dorsal aorta
Stage Range:	20-25 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development