Fig. 10

The YAP/autophagy crosstalk promotes renal interstitial fibrosis during unilateral ureteral obstruction.

a, b Representative images (a) of the kidney cortex of control contralateral kidneys and obstructed kidneys (UUO kidney) 14 days after surgery in Yap^Δtub mice and Yap^flox littermates (PAS staining, original magnification X200) and quantification (b) of the tubular injury score. Data show the mean ± s.e.m.; n = 10 for each experimental group. ANOVA, followed by the two-sided Tukey-Kramer test. c, d Representative images (c) of the kidney cortex of control contralateral kidneys and obstructed kidneys (UUO kidney) 14 days after surgery in Yap^Δtub mice and Yap^flox littermates (picrosirius staining, original magnification X200) and quantification (d) of the interstitial fibrosis area. Data show the mean ± s.e.m.; n = 10 for each experimental group. ANOVA, followed by the two-sided Tukey-Kramer test. e, f Representative images (e) of kidney sections subjected to immunohistochemistry for YAP. Scale bars, 30 µm (f) Quantification of YAP nuclear levels in the renal tubules. Data show the mean ± s.e.m.; n = 5 mice. two-sided t-test. g–i Representative images of control contralateral kidneys and obstructed kidneys (UUO kidney) in Yap^Δtub mice and Yap^flox littermates subjected to immunohistochemistry for LC3. Scale bars, 10 µm. h, i Quantification of LC3 puncta number (h) and size (i) in the renal tubules. Data show the mean ± s.e.m.; n = 5 for each experimental group. two-sided t-test. j, k Representative images (j) of kidney sections subjected to immunohistochemistry for SQSTM1. Scale bars, 10 µm. k Quantification of SQSTM1 puncta in the renal tubules. Data show the mean ± s.e.m.; n = 5 for each experimental group. two-sided t-test. Source data are provided as a Source Data file.