FIGURE

Fig. 4

ID

ZDB-FIG-231201-51

Publication

Li et al., 2023 - FK506-binding protein 5 regulates cell quiescence-proliferation decision in zebrafish epithelium

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Fig. 4

Fkbp5 promotes NaR cell quiescence-proliferation transition via Akt signalling. (A, B) Tg(igfbp5a:GFP) embryos were raised in E3 medium until 3 dpf and transferred to the control (Control) or induction medium (induced). Two days later, they were subjected to phospho-Akt immunostaining. Phospho-Akt-positive cells in the yolk sac region were quantified. Representative images were shown in (A) and quantified data in (B). Data shown are mean ± SD. (C) Effect of myrAkt expression. Tg(igfbp5a:GFP) embryos injected with BAC (igfbp5a:mCherry) or BAC (igfbp5a:myr-Akt-mCherry) were raised. At 3 dpf, they were transferred to the induction medium containing DMSO or 10 mm FK506. At 5 dpf, NaR cells labelled by both GFP and mCherry were scored following a previously established scoring system [[21]]. NaR cells that that did not divide, divided one time or two times are scored as −, + and ++, respectively. *P < 0.05; ****P < 0.0001; ns, not significant. Total cell number is shown above each column.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FEBS Lett.