FIGURE

Fig. 1

ID

ZDB-FIG-231121-52

Publication

Pluimer et al., 2023 - Behavioral analysis through the lifespan of disc1 mutant zebrafish identifies defects in sensorimotor transformation

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Fig. 1

Generation of the disc1 mutant genetic model in zebrafish (A) Representation of the 15 exon disc1 gene. Red asterisk indicates the site targeted. (B) The CRISPR-Cas9 target site is highlighted in yellow, with the PAM underlined. A mutant line with a 20-base pair deletion (denoted by red dashes) was identified. (C) Depiction of the putative Disc1 protein domains. The red line indicates the predicted length of the truncated protein in disc1 mutants. (D) Western blot of Disc1 protein in lysates from disc1 WT and HOM larvae at 7 dpf (25 larvae per lane). (E) Ratio of genotypes in offspring generated by disc1 heterozygous incrosses (n = 451; age = 3 months). (F) Light micrographs of disc1 WT and HOM larvae at 7 dpf. (G) Diagram depicting the timeline of neurobehavioral assays conducted through lifespan.

Expression Data

Antibody:	Ab1-disc1
Fish:	WT disc1^{biz101/biz101}
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	disc1^{biz101/biz101}
Observed In:	whole organism
Stage Range:	Days 7-13 to Adult

Phenotype Detail

Acknowledgments

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