FIGURE

Fig. 2.

ID

ZDB-FIG-231118-19

Publication

Li et al., 2023 - The RNA-binding protein CSDE1 promotes hematopoietic stem and progenitor cell generation via translational control of Wnt signaling

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Fig. 2.

Csde1 regulates HSPC specification in an EC autonomous manner. (A) WISH analysis showing the expression of runx1 (arrowheads) in WT, csde1 mutants and csde1 mutants injected with hsp70: flag-hCSDE1^WT-EGFP or hsp70: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (B) Quantification of the WISH data in A. (C) qPCR analysis of runx1 in WT, csde1 mutants and csde1 mutants injected with hsp70: flag-hCSDE1^WT-EGFP or hsp70: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (D) WISH analysis showing the expression of runx1 (arrowheads) in WT, csde1 mutants and csde1 mutants injected with fli1a: flag-hCSDE1^WT-EGFP or fli1a: flag-hCSDE1^DN-EGFP constructs at 36 hpf. (E) Quantification of the WISH data in D. (F) qPCR analysis of runx1 in WT, csde1 mutants and csde1 mutants injected with fli1a: flag-hCSDE1^WT-EGFP or fli1a: flag-hCSDE1^DN-EGFP constructs at 36 hpf. Data are mean±s.d. **P<0.01, ***P<0.001, ****P<0.0001 (two-tailed unpaired Student's t-test). ns, not significant. n≥3 replicates. Numbers indicate the number of embryos with respective phenotype/total number of embryos analyzed in each experiment (A,D). Scale bars: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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