Defects in protrusion formation and germ cell polarity in sod2 KO and neomycin-treated embryos. Analysis of (A, D) the number of blebs in PGCs per minute and (B, E) the proportion of blebs at the cell back. In graphs A and B, MZsod2STOP31 embryos were injected with control RNA or with RNA directing Sod2 expression to the germ cells (see Methods); the same cells were analyzed in panels A and (B). In graphs D and E, wild-type embryos were treated with DMSO or with neomycin; the same cells were analyzed in panels D and (E). (C, F) Snapshots from the representative time-lapse movies (movies S1 and S2) analyzed in (A,B,D, E). Yellow asterisks point at blebs at the rear of cells. n - the number of PGCs analyzed. One PGC per embryo was imaged and analyzed. **p < 0.01, *p < 0.05, were calculated by Student’s t-test.
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