Fig. 1

Adult zebrafish ligament transection injury and regeneration.

a Schematic of the articulating bones at the zebrafish jaw joint and connections of the interopercular-mandibular (IOM) ligament. b–h Toluidine blue staining of adult zebrafish IOM ligament in uninjured (b, n = 16), 1 day post-ligament transection (dplt, c, n = 3), 3 dplt (d, n = 5), 7 dplt (e, n = 6), 14 dplt (f, n = 16), 21 dplt (g, n = 3), and 28 dplt (h, n = 16). i–n Repeated live imaging of the ventral view of scxa:mCherry expression in IOM ligament before injury (i, n = 9), immediately after injury (j, n = 3), 3 dplt (k, n = 9), 6 dplt (l, n = 3), 14 dplt (m, n = 9), and 28 dplt (n, n = 3). o–r Confocal microscopy imaging of tissue cleared thbs4a_p1:GFP expression in IOM ligament uninjured control (o, n = 6), 1 dplt (p, n = 5), 7 dplt (q, n = 6), and 28 dplt (r, n = 3). AA, anguloarticular bone; IOM, interopercular-mandibular ligament; IOP, interopercular bone; Q, quadrate bone; RA, retroarticular bone. Scale bars = 100 μm (b–h, o–r), 250 μm (i–n).