FIGURE

Figure 5

ID

ZDB-FIG-231002-329

Publication

Zhou et al., 2023 - Inhibition of mitochondrial fatty acid β-oxidation activates mTORC1 pathway and protein synthesis via Gcn5-dependent acetylation of raptor in zebrafish

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Figure 5

Acetyltransferase Gcn5 mediates the regulation of Raptor acetylation in ZFL cells.A–C, the effects of Gcn5 siRNA treatment on global protein lysine acetylation (A), protein quantification of acetyltransferase (P300, Gcn5, and Pcaf) (B), and Raptor acetylation (C). Scale bars represent 5 μm. N = 3. D, the effects of gcn5 siRNA treatment on mTORC1 activity (p-S6^Ser235/236 and p-4EBP^Thr37/46) in ZFL cells. N = 3. E, the effect of gcn5 siRNA treatment on cell growth performance in ZFL cells. N = 3. F, the effect of CPTH6 treatment on the relative growth rate of ZFL cells. N = 7. Data represent mean ± SD. ∗p < 0.05 and ∗∗p < 0.01. Gcn5, general control nondepressible 5; mTORC1, mechanistic target of rapamycin complex 1; Pcaf, P300/Creb-binding protein–associated factor; ZFL, zebrafish liver.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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