FIGURE

Fig. 2

ID
ZDB-FIG-230905-2
Publication
Liu et al., 2023 - Ferroptosis of brain microvascular endothelial cells contributes to hypoxia-induced blood-brain barrier injury
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Hypoxia-induced BBB injury is related to oxidative stress. (A) Quantification of MDA content in the head of zebrafish. N = 150 fish per group. Mean ± SD, Student's t-test, ***p = .0002. (B) 4-HNE and β-tubulin proteins were measured in zebrafish head by Western blot. Mean ± SD, Student's t-test, *p = .0301. N = 100 fish per group. (C) Representative images of bEnd.3 cells stained with ROS assay kit and Hoechst. Mean ± SD, Student's t test, **p = .0011, scale bar: 100 μm. (D) Immunofluorescence staining of CLDN5 and 4-HNE. Mean ± SD, Student's t-test, *p = .0316. (E) Typical bands of 4-HNE and β-tubulin proteins in bEnd.3 cell by Western blot. Mean ± SD, Student's t-test, *p = .0189.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.