FIGURE

Fig. 8

ID

ZDB-FIG-230807-22

Publication

Elworthy et al., 2023 - Activated PI3K Delta Syndrome-1 mutations cause neutrophilia in zebrafish larvae

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Neutrophil migration in pik3cd^E1017K/+ embryos. (A) Illustrative example of the TgBAC(mpx:gfp)i114 tail-fin injury inflammation model used to track neutrophil migration at 3 dpf. Following a minor tail-fin injury and widefield time-lapse microscopy for 6 hpi, semi-automated segmentation and tracking with NIS Elements software determined migration paths to the injury site. Segmented TgBAC(mpx:gfp)i114 neutrophils are shown as false-coloured patches, and a migration track is shown as a green line, superimposed on a brightfield micrograph (taken at the start of the time-lapse sequence). Scale bar: 100 µm. (B) Chart of path speeds for tracked neutrophils from sibling pik3cd^E1017K/+ and pik3cd^+/+ embryos as in A. Data are shown for each of six experiments, each with eight or nine embryos. (C) Chart of pooled data from B. Bars show the mean±s.e.m., unpaired t-test with Welch's correction. (D) Chart of meandering index (displacement/path length) for the tracked neutrophils as in B. (E) Chart of pooled data from D. Bars show the mean±s.e.m., unpaired t-test with Welch's correction.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.