FIGURE

Figure 3

ID
ZDB-FIG-230630-26
Publication
Stemerdink et al., 2023 - Generation and Characterization of a Zebrafish Model for ADGRV1-Associated Retinal Dysfunction Using CRISPR/Cas9 Genome Editing Technology
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Figure 3

Reduced expression of usherin and Whrnb at the photoreceptor periciliary region of adgrv1rmc22 zebrafish larvae. Retinal cryosections of wild-type and adgrv1rmc22 zebrafish larvae (5 dpf) stained with antibodies directed against usherin (red) (A) or Whrnb (red) (B) and centrin (green). Nuclei are counterstained with DAPI (blue). (A): In wild-type larvae, usherin was present at the photoreceptor periciliary region in close proximity to the connecting cilium marker centrin. The intensity of the usherin signal in adgrv1rmc22 retinal sections was significantly reduced when compared to wild-types (n = 7 adgrv1rmc22 mutant larvae and n = 4 wild-type larvae). (B): In wild-type larvae, Whrnb was present at the photoreceptor periciliary region in close proximity to the connecting cilium marker centrin. The intensity of the Whrnb signal in adgrv1rmc22 retinal sections was significantly reduced when compared to wild-types (n = 7 adgrv1rmc22 mutant larvae and n = 6 wild-type larvae). Intensities of fluorescence signals were quantified (mean ± SD) and plotted in a scatter plot next to the corresponding pictures. **** indicates p < 0.0001 (two-tailed unpaired Student’s t-test). Scale bar: 10 μm.

Expression Data
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Day 6

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Day 5 to Day 6

Phenotype Detail
Acknowledgments
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