Fig. 2

Loss of chd7 induces a context‐dependent LLC phenotype in auditory driven behaviors. (A) Acoustic startle responses, average short‐latency c‐bend (SLC) frequency as acoustic stimulus intensity increases (chd7 ^+/+ n = 64, chd7 ^ncu101/+ n = 127, chd7 ^{ncu101/ncu101} n = 61) (mean ± SEM). (B) Short‐latency c‐bend sensitivity index, calculated by the area under the SLC frequency curves for individual larvae (mean ± SD, Ordinary one‐way ANOVA with Tukey's multiple comparison). (C) Long‐latency c‐bend (LLC) frequency as acoustic stimulus intensity increases (mean ± SEM). (D) Long‐latency c‐bend sensitivity index calculated by the area under the LLC frequency curves for individual larvae (mean ± SD, Kruskal‐Wallis with Dunn's multiple comparisons). (E) Short‐term habituation, average SLC frequency during 30 acoustic stimuli at highest intensity (chd7 ^+/+ n = 40, chd7 ^ncu101/+ n = 82, chd7 ^{ncu101/ncu101} n = 47) (mean ± SEM). (F) SLC half‐life calculated by nonlinear regression (one‐phase exponential decay) of SLC frequency curves for individual larvae (mean ± SD, Kruskal‐Wallis with Dunn's multiple comparisons). (G) Average LLC frequency during 30 acoustic stimuli at highest intensity (mean ± SEM). (H) LLC sensitivity index calculated by the area under the LLC frequency curves for individual larvae (mean ± SD, Kruskal‐Wallis with Dunn's multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001).