Fig. 1.
- ID
- ZDB-FIG-230602-4
- Publication
- Wesselman et al., 2023 - Esrrγa regulates nephron and ciliary development by controlling prostaglandin synthesis
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esrrγa is essential for nephrogenesis. (A) Zebrafish nephron development from 20-28 ss. Nephrons possess proximal convoluted tubule (PCT), proximal straight tubule (PST), distal early (DE) and distal late (DL) segments. (B) WISH and FISH reveal that esrrγa transcripts colocalize with renal progenitor marker pax2a at 20 ss. Somites marked with smyhc (red). At 28 ss, esrrγa colocalizes with tubule marker cdh17. (C) esrrγa morphants display pericardial edema (asterisk), smaller eyes and altered head morphology (arrowhead) and fused otoliths (inset) (left). Dextran-FITC in the PCT at 48 hpi, white dotted line outlines nephron (right). (D) WT and esrrγa SB morphant at 6 hpi and 48 hpi after dextran-FITC. (E,F) WISH for PST marker trpm7 (E) and DL marker slc12a3 (F) with somite marker smyhc or nephron marker cdh17. (G,H) Length of PST (G) and DL (H) at 28 ss, each dot represents a single animal. (I) WISH for MCC marker odf3b at 24 hpf. (J) MCCs per nephron; each dot represents one nephron. Two nephrons were counted per animal. (K) FISH for MCCs (odf3b, green and dashed ovals), PST cells (trpm7, red) and DNA (DAPI, blue) at 24 hpf. (L) Absolute cell number of odf3b- and trpm7-expressing cells at 24 hpf. Each dot represents a single animal. Data are mean±s.d. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 (unpaired t-test or one-way ANOVA). ns, not significant. Scale bars: 100 µm (B-F); 50 µm (B,C,E,F insets, I,K). |