FIGURE

Figure 3.

ID

ZDB-FIG-230523-26

Publication

Cevik et al., 2023 - WDR31 displays functional redundancy with GTPase-activating proteins (GAPs) ELMOD and RP2 in regulating IFT complex and recruiting the BBSome to cilium

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Figure 3.

WDR31 regulates ciliogenesis in zebrafish.

(A) Shown is the expression pattern analysis of wdr31 in zebrafish embryos. wdr31 is ubiquitously expressed before the segmentation stage (two-cell and eight-somite stages [SS]). The expression of wdr31 becomes limited to the otic vesicle and brain region at 24 hours post fertilization (24 hpf). (B) Shown are the cilia of lateral crista (LC) of the otic vesicle, stained with acetylated-tubulin, in WT and wdr31 knockout, generated via CRISPR/Cas9. (C, D) The length of cilia in the LC of the otic vesicle remains unaffected in zebrafish wdr31 knockout, whereas the cilia number is decreased, as shown with the measurement of width of cilia LC of the otic vesicle.

Expression Data

Gene:	wdr31
Fish:	WT
Anatomical Terms:	brain otic vesicle whole organism
Stage Range:	2-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-wdr31 + CRISPR2-wdr31
Knockdown Reagents:	CRISPR1-wdr31 CRISPR2-wdr31
Observed In:	lateral crista cilium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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