Fig. 4

Figure 4. Ubiquitous overexpression of cytosolic hSNRNP70-eGFP partially restores motor neuron growth and neuromuscular assembly

(A) Ubiquitous overexpression of cytosolic hSNRNP70-eGFP using the Tg(ubi:ERT2-Gal4;UAS:hSNRNP70ΔNLS3xNES-eGFP) line. Embryos derived from the same clutch were sorted into four groups according to genotype: (1) sib, (2) sib/cyt-hSNRNP70, (3) null, and (4) null/cyt-hSNRNP70. Representative images of live embryos at 48 hpf from three independent experiments. Images are lateral views with anterior to the left.

(B) Maximum intensity projection images of trunk, lateral views with anterior to the left, at 28 hpf stained with anti-SV2 antibody to mark the pre-synaptic locations and a fluorescently tagged α-BTX to label AChRs on post-synaptic structures. Representative images from three independent experiments. Scale bars, 25 μm.

(C) Quantification of touch-evoked startle response at 48 hpf. The graph shows mean values ± SEM. ^∗∗∗∗p < 0.0001; ^∗∗p < 0.01; ^∗p < 0.05; ns, not significant, Kruskal-Wallis test, n = 12 animals per group in three independent experiments.

(D) Quantification of the degree of overlap between SV2 and α-BTX at 28 hpf in the four groups. The graph shows mean values ± SEM. ^∗∗∗∗p < 0.0001; ^∗∗p < 0.01; ns, not significant, one-way ANOVA, n = 12 animals per group in three independent experiments.

(E) Quantification of motor nerve length at 28 hpf. The graph shows mean values ± SEM. ^∗∗∗∗p < 0.0001; ^∗∗p < 0.01; ^∗p < 0.05; ns, not significant, one-way ANOVA, n = 12 animals per group in three independent experiments.

See also Figures S4 and S5.