FIGURE

Figure 3

ID

ZDB-FIG-230228-132

Publication

Wesselman et al., 2023 - Estrogen Signaling Influences Nephron Segmentation of the Zebrafish Embryonic Kidney

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Figure 3

Chemical and genetic inhibition of Esr2b results in altered distal segmentation. (A) The 28 ss WT (top) and 18 µM PHTPP (bottom) treated animals stained via WISH for the DE marker (slc12a1) (left) or the DL marker (slc12a3) (right). Scale bar = 100 µm for lower magnification, scale bar = 50 µm for higher magnification. (B,C) DE and DL domain lengths at 28 ss in micrometers. (D) The 28 ss WT (top), esr2b MO-injected (second row), esr2a and esr2b double-MO-injected (third row), and esr2b mutant (bottom row) animals stained via WISH for the DE marker (slc12a1) (left) or the DL marker (slc12a3) (right). Scale bar = 100 µm for lower magnification, scale bar = 50 µm for higher magnification. (E,F) DE and DL domain lengths at 28 ss in micrometers. Data presented on graphs are represented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (t-test or ANOVA).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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