Figure 4

The lama2^re16, lrrc4ca^re17lrrc4cb^re18, and kcnq5a^re19kcnq5b^re20 mutants showed an increased axial length. (a) Single B-scan image of a typical 4mpf WT zebrafish eye. The axial length (blue) spans from the apical part of the corneal epithelium to the anterior border of the RPE. The gradient refractive index of the spherical zebrafish lens was used as a correction factor to acquire this image (see “Methods”). Individual compartments: cornea and anterior chamber (red), lens (yellow), vitreous chamber (orange), neural retina (magenta) and RPE (green). (b) Axial length of size-matched 2 and 4mpf zebrafish measured by spectral-domain optical coherence tomography. At 2mpf an increase in axial length was found for lrrc4c mutant (Effect size = 140.0 μm, p < 0.0001) and kcnq5 mutant (Effect size = 151.0 μm, p < 0,0001) relative to WT fish. At 4mpf a relative increase was found for lama2 mutant (Effect size = 69.00 μm, p = 0.0083), lrrc4c mutant (Effect size = 114.0 μm, p < 0.0001), and kcnq5 mutant (Effect size = 92.00 μm, p = 0.0044). See Supplementary Data ^S4 for all data. Error bars: SEM. Significance: ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Mpf: months post-fertilization, RPE: retinal pigmented epithelium, WT: wild-type, tjp2 mutant: tjp2a^b1367tjp2b^b1368, pde11a mutant: pde11a^re13pde11a-like^re14, shisa6 mutant: shisa6^re15, lama2 mutant: lama2^re16, lrrc4c mutant: lrrc4ca^re17lrrc4cb^re18, kcnq5 mutant: kcnq5a^re19kcnq5b^re20, SEM: Standard error of the mean.